On 31 August 2016 the autonomous Community of Madrid, Spain, reported two cases of infection with Crimean–Congo haemorrhagic fever(CCHF) virus.

The Federal Office for Public Health Safety has been informed by the Italian authority that WNV NAT testing will now be carried out in the province of Vercelli (Piedmont Region) in addition to the regions already announced.

The Federal Office for Safety in Health Care has been informed by the Italian authority that WNV NAT testing will now be carried out in the province of Vicenza (Veneto Region) in addition to the regions already announced.

Given the current circulation of Zika virus ,the evidence of an association between Zika virus infection during pregnancy and congenital malformations of the central nervous system (CNS), the association between Zikavirus infection and Guillain–Barré syndrome(GBS)and the risk of local vector-borne transmission in Europe during the 2016 summer season, EU/EEA Member States should consider a range of mitigation measures.

The Federal Office for Safety in Health Care has been informed by the Italian authority that WNV NAT testing will now be carried out in the province of Milano (Lombardia Region) in addition to the regions already announced.

The Federal Office for Public Health Safety has been informed by the Greek authority that four confirmed locally acquired malaria cases have been recorded in Greece (two in the western Peloponnese and two in central Macedonia).

The Federal Office for Public Health Safety has been informed by the Italian authority that WNV NAT testing will now be carried out in the province of Ogliastra (Region of Sardinia) in addition to the regions already announced.

The Federal Office for Public Health Safety has been informed by the Italian authority that WNV NAT testing will now be carried out in the provinces of Pavia and Lodi (Lombardia Region) in addition to the regions already announced.

The Federal Office for Safety in Health Care was informed by the Italian authority that two blood donors in the provinces of Mantova and Cremona (Lombardia Region) tested positive for WNV NAT. Furthermore, four symptomatic human WNV cases (two of which were neuroinvasive) were reported in the provinces of Verona, Rovigo (Veneto Region), and Reggio Emilia (Emilia Romagna Region).

The Federal Office for Safety in Health Care has been informed by the Italian authority that WNV NAT testing will now be carried out in the province of Padova (Veneto Region) in addition to the regions already announced.